The FACSLyric from Waters Biosciences (former BD Biosciences) is a 3-laser flow cytometer that is configured with 12 independent photomultipliers. It is a high-performance, highly sensitive flow cytometer with exceptional resolution, with up to 35.000 events/sec.

LaserParameterFilterCommon dyes/fluorochromes*



405nm

E

448/45

BV421, Alexa Fluor 405, V450, Pacific Blue, VioBlue, Live/Dead Violet

D

528/45

BV510, Alexa Fluor 430, V500, Amcyan, VioGreen, Live/Dead Aqua

C

606/36

BV605, Qdot 605

B

715/50

BV711, Qdot 705, BV750

A

755 LP

BV750, BV785, BV786, Qdot 800

488nm

D

527/32

FITC, Alexa Fluor 488, BB515, Live/Dead Green

C

586/42

PE, Spark Blue 585, PI

B

700/54

PerCP, PerCP-Cy5.5, PE-Cy5, BB700, 7-AAD

A

786/46

PE-Cy7, PE-Vio770

633nm

C

660/20

APC, Alexa Fluor 647, Live/Dead Far Red

B

720/30

APC-R700

A

783/56

APC-Cy7, APC-H7, APC-Alexa Fluor 750, APC-Vio770, Live/Dead Near-IR

* This is a list of fluorochromes commonly used on the FACSLyric. The list is not exhaustive, if you would like to use a different fluorochrome, please contact the facility.