Introduction to flow cytometry
Flow cytometry is a powerful technique for the multi-parametric analysis of individual cells within heterogeneous populations. Our facility currently offers a wide range of flow cytometers, from basic flow cytometers to an advanced spectral flow cytometer capable of distinguishing up to 40 - 50 colors simultaneously. This technology enables rapid analysis (within seconds to minutes) of large numbers of cells (10⁴–10⁶), providing high-resolution insights into complex biological systems
Possible applications
- Analysis of intra- and extracellular antigens by means of immune fluorescence
- Cell cycle and DNA ploidy analysis
- Cell proliferation and cell death detection
- Absolute or relative cell counts
- Membrane potential
- Phagocytosis
- Monitoring of gene transfer
- Intracellular Ca2+ and pH responses
- ...